Characterization of Nisin Produced by Lactococcus lactis RP359 Isolated from Kem-Buk-Nud, a Traditional Thai Fermented Food
نویسنده
چکیده
Five hundred colonies of lactic acid bacteria (LAB) isolated from Kem-Buk-Nud, a traditional Thai fermented food, were screened for the presence of nisin gene by using the polymerase chain reaction (PCR) with primers specific to nisin A structural gene. A LAB isolate, RP359, identified as Lactococus lactis subsp. lactis was found to give an expected 231-bp PCR product and to have antimicrobial activity. The inhibition zones produced by RP359 nisin were larger than those produced by nisin A. Sequencing of the PCR product showed that the gene encoding RP359 nisin was a nisin A gene with a silent mutation. The relationship between the mutation and the production of the larger inhibition zones was discussed. To my knowledge, this is the first report presenting the variant of nisin A gene with silent mutation.
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